WebMar 22, 2024 · In brief, for active caspase-3 assay, cells were fixed in BD Cytofix/Cytoperm solution, incubated on ice for 20 min, washed with BD Perm/Wash buffer, and then incubated for 30 min at 25°C with FITC rabbit anti-active caspase-3 antibody. WebMay 3, 2000 · The presence of a low amount of active caspase 3 in the treated viable cells was confirmed in situ with PE-conjugated anti–active caspase 3 antibodies. The same antibody was used in combination with FITC-annexin V and CD45–PC5 to study caspase 3 activation in acute leukemia blast cells after in vitro DNR and IDA treatment.
TLR3 Expression Induces Apoptosis in Human Non-Small-Cell …
WebThe processed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active Caspase 3 … WebProduct name: FITC Rabbit Anti-Active Caspase-3 (51-68654X) (Contd. of page 1) 33.1 · After swallowing If symptoms persist consult doctor. 5 Firefighting measures · Suitable extinguishing agents CO2, ABC multipurpose dry chemical or water spray. Fight larger fires with water spray or alcohol resistant foam. flyhigh dresses
Anti-Caspase3 (active form) Antibody, clone 3D9.3 - Sigma-Aldrich
http://pufei.com/static/product/bd/550/1505231617e995b8.pdf WebRabbit Anti Human Active Caspase 3 Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ... FITC-conjugated goat anti-rabbit and Texas Red-conjugated horse anti-mouse IgGs from Vector (Burlingame, CA, USA); HRP-conjugated donkey anti-rabbit and … WebJul 1, 2014 · The presence of active caspase-3 using FITC Active Caspase-3 Apoptosis Kit. Cells were plated in 60 mm culture dish at a seeding density of 5 × 10 5 cells/dish. GTT or HC was dissolved in ethanol, added to the culture media to the specified final concentration. ... washed with BD Perm/Wash buffer and FITC rabbit anti-active … fly high english translation