WebA Versatile Workflow for the Identification of Protein-Protein Interactions Using GFP-Trap Beads and Mass Spectrometry-Based Label-Free Quantification Methods Mol Biol. 2024 ... Enrichment of the GFP-tagged protein together with its interaction partners, as well as the free GFP, is performed by immunoaffinity purification. The pull-down quality ... WebProduct Information. GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads. Description. …
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WebMar 13, 2024 · GFP-NbPP1C was used as a positive control, and GFP-GUS and RFP-GUS were used as negative controls. IP samples were plant lysates incubated with GFP-Trap beads. NbCLC2-GFP, NbAra6-GFP, GFP-NbPP1C, and GFP-GUS were detected with an antibody against GFP (α-GFP), and Pi04314-mRFP and RFP-GUS were detected with … WebThe ChromoTek GFP-Trap®Dynabeads™ consists of an anti-Green Fluorescent Protein (GFP) Nanobody (VHH), which is covalently bound to Dynabeads. GFP-Trap Dynabeads … pheno font
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WebMay 28, 2024 · A Versatile Workflow for the Identification of Protein–Protein Interactions Using GFP-Trap Beads and Mass Spectrometry-Based Label-Free Quantification Guillaume Née, Priyadarshini Tilak & Iris Finkemeier Protocol First Online: 28 May 2024 1137 Accesses 2 Citations Part of the Methods in Molecular Biology book series … WebApr 12, 2024 · HIGHLIGHTS who: Ashima Mohanty and collaborators from the Division of Genomics and Precision Medicine, College of Health and Life Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation, Doha POBox, … The yeast permease agp2 senses cycloheximide and undergoes degradation that requires the small protein brp1 … WebThe µMACS and MultiMACS GFP Isolation Kits facilitate the isolation of GFP fusion proteins from different sources. The super-paramagnetic µMACS MicroBeads included in the kits are conjugated to an anti-GFP monoclonal antibody, enabling fast and effective magnetic labeling of GFP-tagged fusion proteins. phenograph r语言