Phis2.1 载体图谱

Author: fuyc

August undefined, 2024

Webb6 feb. 2024 · Lower panel revealed that pHis2.1 vector encompassing FL, ∆1, or ∆2 promoter region of the ANAC003 gene was amplified, respectively by using the primer set located at the pHis2.1 vector. Webb6 sep. 2024 · 1）pHis2-bait和AD-prey质粒混合作为实验组，将pHis2-bait和空载AD质粒混合作为自激活组。将实验组和自激活组分别共转到Y187感受态细胞中。 2）用SD/-Leu/ …

phis2.1载体图 pHis2酵母单杂交载体 - 无锡文化网

http://www.biofeng.com/zaiti/jiaomu/pHis2.html Webb6 feb. 2024 · The chloroplast comprises the outer and inner membranes that are composed of the translocon protein complexes Toc and Tic (translocon at the outer/inner envelope membrane of chloroplasts), respectively. Tic55, a chloroplast Tic protein member, was shown to be not vital for functional protein import in Arabidopsis from previous … dr kearney cocoa village

PT3705-5 (PR32049) pHIS2

WebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … WebbpHIS2.1 DNA target TRP1 Kanr HIS3 Figure 2. One-hybrid library construction and screening. Your target bait sequence is cloned upstream of the His3 reporter in pHIS2.1. … Webb19 nov. 2012 · 我把要验证的转录因子构建到pGADT7-rec2载体上，与pHis2的空载共同转化Y187，本来要拿这个做阴性对照的，可是在三缺板子上长得很欢乐呀，加了3AT，并且 … cohesion forum

Phis2.1 载体图谱

WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community …

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Webb1 juni 2024 · Digest the pHIS2 vector with Sma I purify it by agarose gel electrophoresis (0.8% agarose). 5. For addition of a single ‘T’ base at the ends of the linearized pHIS2 plasmid, prepare the following mix in a 10 μL total volume: 1 μg pHIS2 ( Sma I linearized), 0.5 μL dTTP (stock concentration 10 mM), 1 μL 10× ExTaq buffer, 0.5 U ExTaq. http://www.bioon.com.cn/reagent/show_product.asp?id=3900880

WebbVector Database. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. Only the plasmids deposited at Addgene are available for purchase through this website. WebbpHis2载体价格650元，pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Kanamycin，质粒大小为7207bp。pHis2是酵母单杂交载体。

http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627 WebbpHISi is a yeast integration and reporter vector for use with the MATCHMAKER One-Hybrid System. pHISi contains the yeast HIS3 gene downstream of the MCS and the minimal …

Webb1 mars 2024 · C. Yeast one-hybrid assay detect the specific binding ability of VvWRKY30 to W-BOX. pGADT7-empty and pHIS2.1-W-BOX, AD- VvWRKY30 and pHIS2.1-empty were …

WebbpHis2酵母表达载体说明- HaleWaihonaPukeBaidu 载体序列 ORIGIN 1 GAATTCCCGG GGAGCTCACG CGTTCGCGAA TCGATCCGCG GTCTAGAAAT ... pFLD/CAT pFLDα pGADT7-T pGAG424 pGAPZA pGAPZB pGAPZαB pGAPZαC pGBKT7 pGBKT7-53 pGBKT7-Lam pHIC-PI pHIL-D2 pHIL-S1 pHis2 pHisSi-1 pMETA pMETB pMETC pMETαA pMETαB pMETαC … dr kearney middle schoolWebbDifferent pairs of plasmids (pGAD-OsWRKY54 and pHIS2- ProOsHKT1;5 , or control pGADT7 and pHIS2) were transformed into yeast cells. Transformed cells were spotted on SD medium containing 0, 50, or 75 mM 3-amino-1,2,4-triazole at 30 °C for 3 … dr kearney dentist cary ncWebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... cohesion for rockWebbPhis2osgpx1 Promoter Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > phis2 1. phis2 1 vector TaKaRa is a verified supplier TaKaRa ... cohesion freight agencyWebb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in plant resistance to various external stresses. However, functional characterization of tea plant (Camellia sinensis L.) CBF gene (CsCBF) was still seldom reported. Here, functional … dr kearney pediatricianWebb使用phis2载体，将启动子序列构建在his3报告基因前面的多克隆位点上，对应的转录因子构建在ad载体上即可。使用Y187菌株，它相对于AH109菌株在基因组上少了HIS的报告基 … cohesion freight agency ltd shanghai branchWebb16 jan. 2015 · 广东汕尾市海丰县自然资源局公开招聘政府聘员1人【含答案解析】模拟试卷8 未来工匠我来说演讲稿范文（5篇） 2024年山东潍坊寿光市事业单位招考聘用硕士研究生30人【答案解析】模拟试卷5 cohesion fundraising