WebR-Evolution by Rieker . NEW Samsung Phones. HUGE Discount . Compact Blood Pressure Monitor ... 50% Off HVAC service plans and WIN A FREE FURNACE "Wuru" Wool Premium … WebApr 10, 2024 · The cell pellet was resuspended in Buffer A (50 mM Tris-Cl, pH 8.0, 150 mM NaCl), and cells were lysed via sonication on ice for 5 min for a total of three times. The sample was centrifuged at 8000× g at 4 °C for 15 min, and the supernatant was loaded onto Strep-Tactin TM resin (IBA Life Sciences, Göttingen, Germany). The resin was washed ...

Strep-Tactin® Sepharose® 50% suspension - Neuromics

WebMar 3, 2024 · Add 15 µL of Strep-Tactin Sepharose 50% suspension to the lysate and rotate at 4 °C for 1 h. × g for 1 min at 4 °C. Aspirate the supernatant. Add 1 mL of cold pull-down washing buffer to each tube and mix via inversion. Repeat steps 28 … Web3 Columns: StrepTrap XT 1 mL StrepTrap HP 1 mL Sample: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH Strep-tag II, tetramer, M r 37 400/subunit), 1 mg/mL in E. coli lysate Sample volume: 3 mL Binding buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8.0 Elution buffer: StrepTrap XT column: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 50 mM … cshelh-st3w-m8-14

口腔医学小站 – 第 2 页 – 口腔文献阅读与资源分享

http://www.dentalearner.com/page/2 WebDescription Immobilized streptavidin variant called Strep-Tactin® (5 mg/ml resin) which has been especially optimized for the purification of Strep-tag®II fusion proteins*. Support … WebUS20240087953A1 US17/799,239 US202417799239A US2024087953A1 US 20240087953 A1 US20240087953 A1 US 20240087953A1 US 202417799239 A US202417799239 A US 202417799239A US 2024087953 A cshelh-st3w-m8-16